產(chǎn)品詳情介紹
TransScript? Uni All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)包含反轉(zhuǎn)錄反應(yīng)所需的全部成分(TransScript? Uni RT, RNase Inhibitor, Anchored Oligo(dT)20 Primer, Random Primer(N9), dNTPs, Buffer)，可在同一管反應(yīng)中實(shí)現(xiàn)cDNA 合成和去除基因組DNA。反應(yīng)時，只需加入5×All-in-One Reaction Mix for qPCR、TransScript? Uni All-in-One Enzyme Mix、模板RNA和水，在42℃-65℃條件下即可高效地合成第一鏈cDNA，同時去除RNA模板中殘留的基因組DNA 。另配有20×No RT Control Mix，用于配制無反轉(zhuǎn)錄酶的對照，判斷qPCR模板是否來自cDNA。該產(chǎn)品操作簡便，降低操作過程中的污染機(jī)率。cDNA產(chǎn)物只適用于qPCR，不適用于常規(guī)PCR。
1 Qin T, Zhang G, Zheng Y, et al. A population of stem cells with strong regenerative potential discovered in deer antlers[J]. Science, 2023.(IF 63.71)
2 Yang L, Wu X, Liu S, et al. Comprehensive Analysis of BrHMPs Reveals Potential Roles in Abiotic Stress Tolerance and Pollen–Stigma Interaction in Brassica rapa[J]. Cells, 2023.(IF 7.66)
3 Yang X, Liu S, Lu W, et al. Delta and jagged are candidate target genes of RNAi biopesticides for the control of Nilaparvata lugens[J]. Frontiers in Bioengineering and Biotechnology, 2022.(IF 6.064)
4 Yuan Y, Fu D, Xu Y, et al. Pt (IV) Prodrug as a Potential Antitumor Agent with APE1 Inhibitory Activity[J]. Journal of Medicinal Chemistry, 2022.(IF 8.039)
